Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters
Open Access
- 1 January 1975
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (7) , 1163-1176
- https://doi.org/10.1093/nar/2.7.1163
Abstract
The level of deoxyadenylate (dA) regions in human DNA was estimated from formation of poly(U)-poly(dA) triplexes on nitrocellulose filters that were RNAase resistant. The (dA) rich sequences were determined following mild ribonuclease treatment of the poly(U)-DNA hybrids (5 μg/ml at 25°C for 30 min), where as exhaustive ribonuclease treatment (5 μg/ml at 25°C for 6 hr) estimated the more (dA) pure sequences. The level of (dA) rich regions was 0.39% of the DNA and for the more (dA) pure regions it was 0.07%.Keywords
This publication has 24 references indexed in Scilit:
- Distribution of Repetitive and Nonrepetitive Sequence Transcripts in HeLa mRNAProceedings of the National Academy of Sciences, 1974
- Differential Metabolism of Large and Small Poly(A) Sequences in the Heterogeneous Nuclear RNA of HeLa CellsProceedings of the National Academy of Sciences, 1974
- Nonrepetitive DNA Sequence Representation in Sea Urchin Embryo Messenger RNAProceedings of the National Academy of Sciences, 1973
- Synthesis of a Deoxyribonucleic Acid Sequence Complementary to Ovalbumin Messenger Ribonucleic Acid and Quantification of Ovalbumin GenesJournal of Biological Chemistry, 1973
- Occurrence of Uridylate-Rich Oligonucleotide Regions in Heterogeneous Nuclear RNA of HeLa CellsProceedings of the National Academy of Sciences, 1972
- Poly U Tracts absent from Viral RNANature New Biology, 1972
- The genes for silk fibroin in Bombyx moriJournal of Molecular Biology, 1972
- Isolation and Hybridization Kinetics of Messenger RNA from Dictyostelium discoideumNature New Biology, 1972
- Polyadenylic Acid Sequences in the Heterogeneous Nuclear RNA and Rapidly-Labeled Polyribosomal RNA of HeLa Cells: Possible Evidence for a Precursor RelationshipProceedings of the National Academy of Sciences, 1971
- A Polynucleotide Segment Rich in Adenylic Acid in the Rapidly-Labeled Polyribosomal RNA Component of Mouse Sarcoma 180 Ascites CellsProceedings of the National Academy of Sciences, 1971