Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.
- 1 March 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (3) , 1441-1445
- https://doi.org/10.1073/pnas.78.3.1441
Abstract
The complete nucleotide sequence of the thymidine kinase (ATP:thymidine 5'' phosphotransferase, EC 2.7.1.21) gene of herpes simplex virus type 1 strain CL101 was determined from a plasmid clone of viral DNA derived by Enquist et al. (1979). A c(complementary)DNA copy of the 5'' end of tyrmidine kinase mRNA was also analyzed to locate the transcribed sequences. The transcribed portion of the gene is .apprx. 1300 nucleotides in length and appears to contain no intervening sequences. These is an untranslated region of 107 nucleotides at the 5'' end of the mRNA followed by an open reading frame of 1128 nucleotides. The gene is thus capable of coding for a protein of 376 amino acids. Sequences similar to those thought to be involved in control of transcription and translation of a variety of eukaryotic and viral genes such as a Hogness box and A-A-T-A-A-A polyadenylylation signals are also present in the herpesvirus thymidine kinase gene.This publication has 34 references indexed in Scilit:
- Promoter Sequences of Eukaryotic Protein-Coding GenesScience, 1980
- Construction in vitro and rescue of a thymidine kinase-deficient deletion mutation of herpes simplex virusNature, 1980
- Construction and characterization of a recombinant plasmid encoding the gene for the thymidine kinase of herpes simplex type 1 virusGene, 1979
- How do eucaryotic ribosomes select initiation regions in messenger RNA?Cell, 1978
- A restriction endonuclease cleavage map of G4Virology, 1978
- Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sitesNature, 1978
- Conservation of the primary structure at the 3′ end of 18S rRNA from eucaryotic cellsCell, 1978
- Transfer of purified herpes virus thymidine kinase gene to cultured mouse cellsCell, 1977
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976
- Herpes Simplex Virus-specific Polypeptides Studied by Polyacrylamide Gel Electrophoresis of Immune PrecipitatesJournal of General Virology, 1974