Preliminary results obtained with a No-Label, Homogeneous, Optical Immunoassay for Human Immunoglobulin G

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Abstract
A novel optical immunoassay system was developed and tested using human IgG as a model antigen. Following adsorption of antiserum to the surface of an optical waveguide, the immobilized antibody was then reacted with a solution containing antigen. The reaction was detected utilizing the evanescent wave component of a light beam totally internally reflected within the waveguide. The growing antigen-antibody layer resulted in an increase of scattered light which was monitored kinetically. Individual measurements were completed within 5 min, and the resultant dose response curve had a sensitivity limit of .apprx. 10 nmol/l.