BACTERIAL METABOLISM THE INFLUENCE OF PHOSPHATE BUFFER IN CARBOHYDRATE-FREE AND IN GLUCOSE-CONTAINING MEDIA

Publisher Website
Google Scholar
Abstract
Phosphate buffer when present in reasonable amount (1%) will in many instances maintain a sufficiently low p H level to permit the given organisms to bring about nitrogenous changes in the substrate as though no utilizable carbohydrate were present. Although phosphate buffer accelerated the growth and metabolic activities of many of the organisms, it did not affect all members of the same group or even closely related species, in the same manner. Metabolic changes in the cultures were followed by comparative determinations of total, non-protein, amino, and ammonia N, as well as of p H, glucose and turbidity. Aside from the influence of buffer on N metabolism, it was hoped to make the study of interest by furnishing definite information on the activities of 22 well-known species, not only as to N changes, but as to the action of these organisms on glucose under different experimental conditions.