Tissue pyridoxal phosphate concentration and pyridoxaminephosphate oxidase activity in riboflavin deficiency in rats and man

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Abstract
1. Parenteral administration of pyridoxal 5′-phosphate (PALP) to riboflavin-deficient rats increased the non-FAD component of erythrocyte riboflavin.2. Pyridoxaminephosphate oxidase (EC1.4.3.5) activity in the livers of riboflavin-deficient animals was only 15% of that of controls. PALP concentration in blood, liver and brain was not affected. Deficient animals had higher levels of pyridoxine in liver.3. Human subjects with lesions of the mouth responded to treatment with either riboflavin or pyridoxine.4. PALP concentration of human blood was not affected by administration of riboflavin but was markedly increased by pyridoxine.5. Erythrocyte glutathione reductase activity (EC1.6.4.2) in humans was increased andin vitrostimulation of the enzyme with FAD was decreased by treatment with riboflavin, but not by treatment with pyridoxine.