Minimisation of bilirubin interference in the determination of fluorescein using first-derivative synchronous excitation fluorescence spectroscopy
- 1 January 1986
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 111 (2) , 205-207
- https://doi.org/10.1039/an9861100205
Abstract
The use of first-derivative synchronous excitation fluorescence is described for the minimisation of bilirubin interference in the determination of fluorescein. The limits of detection and the standard errors of estimates are better using the synchronous first-derivative approach than with conventional subtraction of the bilirubin blank. Results are shown for the quantitation of fluorescein in the nanomole concentration range.Keywords
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