An improved enzyme-linked immunoassay for the detection and quantification of the entomocidal parasporal crystal proteins ofBacillus thuringiensissubspp.kurstakiandisraelensis
- 1 December 1984
- journal article
- research article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 57 (3) , 447-454
- https://doi.org/10.1111/j.1365-2672.1984.tb01410.x
Abstract
An improved and simplified enzyme-linke immunosorbent assay (ELISA) was developed for the detection and quantification of parasporal crystalline toxins from B. thuringiensis ssp. kurstaki. The improved procedure involved pretreatment of the polystyrene cuvettes with glutaraldehyde before antibody coating. A direct comparison of treated and untreated cuvettes is provided. ELISA were then used for the analysis of the entomocidal crystalline proteins in commercial and experimental formulations of B. t. ssp. kurstaki and B. t. israelensis.This publication has 19 references indexed in Scilit:
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