EXPOSURE OF THE RH0(D) ANTIGEN ON THE SURFACE AND CYTOPLASMIC DOMAINS OF THE RED-CELL MEMBRANE

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 45  (1) , 27-30
Abstract
Inside-out (IO) and right-side-out (RO) vesicles derived from human red blood cells were tested for their ability to bind 125I-labeled IgG anti-Rho(D). The binding of anti-Rho(D) to RO vesicles from Rho(D)-positive cells was quantitatively similar to that exhibited by intact cells when compared on a membrane surface area basis. There was no significant binding of labeled antibody to IO vesicles from Rho(D)-positive cells or either RO or IO vesicles derived from Rho(D)-negative cells. The Rho(D) antigen was immunologically accessible on only the plasma side of the membrane in Rho(D)-positive red cells, as shown for blood group antigens defined by carbohydrate determinants. No immunologically reactive Rho(D) antigen was present on either RO or IO vesicles derived from Rho(D)-negative red cells.