Free radical production during phenylhydrazine-induced hemolysis

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Abstract
The production of phenyl radicals during phenylhydrazine-induced hemolysis has been demonstrated by the use of the spin traps, DMPO, M4PO, and LINPyBN. The phenyl spin adducts of DMPO and M4PO were produced in moderate yield by an oxygenated 1% erythrocyte suspension. With the lipid soluble spin trap LINPyBN, a dramatic increase (400%) in the yield of phenyl spin adduct was observed despite little increase in the rate of phenyl radical trapping. The production of phenyl spin adducts was decreased when phenylhydrazine-4-sulphonic acid or carbonmonoxyhemoglobin-containing erythrocytes were used. These results suggest that the reaction of phenylhydrazine with oxyhemoglobin leads to the formation of phenyl radicals that are preferentially trapped in the erythrocyte membrane.

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