Kinetic parameters and renal clearances of plasma adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate in man
Open Access
- 1 December 1970
- journal article
- Published by American Society for Clinical Investigation in Journal of Clinical Investigation
- Vol. 49 (12) , 2222-2236
- https://doi.org/10.1172/jci106441
Abstract
Kinetic parameters and the renal clearances of plasma adenosine 3',5'-monophosphate (cyclic AMP) and guanosine 3',5'-monophosphate (cyclic GMP) were evaluated in normal subjects using tritium-labeled cyclic nucleotides. Each tracer was administered both by single, rapid intravenous injection and by constant intravenous infusion, and the specific activities of the cyclic nucleotides in plasma and urine were determined. Both cyclic AMP and cyclic GMP were cleared from plasma by glomerular filtration. The kidney was found to add a variable quantity of endogenous cyclic AMP to the tubular urine, amounting to an average of approximately one-third of the total level of cyclic AMP excreted. Plasma was the source of virtually all of the cyclic GMP excreted. Plasma levels of the cyclic nucleotides appeared to be in dynamic steady state. The apparent volumes of distribution of both nucleotides exceeded extracellular fluid volume, averaging 27 and 38% of body weight for cyclic AMP and cyclic GMP, respectively. Plasma production rates ranged from 9 to 17 nmoles/min for cyclic AMP and from 7 to 13 nmoles/min for cyclic GMP. Plasma clearance rates averaged 668 ml/min for cyclic AMP and 855 ml/min for cyclic GMP. Approximately 85% of the elimination of the cyclic nucleotides from the circulation was due to extrarenal clearance.Keywords
This publication has 25 references indexed in Scilit:
- Effects of parathyroid hormone on plasma and urinary adenosine 3′,5′-monophosphate in manJournal of Clinical Investigation, 1970
- Effects of glucagon on adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate in human plasma and urineJournal of Clinical Investigation, 1970
- Effect of Mode of Administration on Drug Distribution in a Two-Compartment Open SystemJournal of Pharmaceutical Sciences, 1969
- Membrane ultrafiltration: The diafiltration technique and its application to microsolute exchange and binding phenomenaAnalytical Biochemistry, 1968
- Renal Adenyl Cyclase: Anatomically Separate Sites for Parathyroid Hormone and VasopressinScience, 1968
- Concept of a Volume of Distribution and Possible Errors in Evaluation of This ParameterJournal of Pharmaceutical Sciences, 1968
- Organophosphates of urine, including adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphateBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- Isolation of adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate from rat urineBiochemical and Biophysical Research Communications, 1963
- A new method for the determination of inulin in plasma and urineClinica Chimica Acta; International Journal of Clinical Chemistry, 1956
- THE VELOCITY OF DISTRIBUTION OF SUCROSE BETWEEN PLASMA AND INTERSTITIAL FLUID, WITH REFERENCE TO THE USE OF SUCROSE FOR THE MEASUREMENT OF EXTRACELLULAR FLUID IN MAN 1Journal of Clinical Investigation, 1951