Determination of Benzo[a]pyrene in lipid‐soluble liquid smoke (LSLS) by HPLC‐FL

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Abstract
We developed a method for the determination of low levels of the carcinogen benzo[a]pyrene (BaP) in lipid‐soluble liquid smoke (LSLS). The method consists of partitioning the LSLS (2 g) between n‐hexane and DMSO, purification of the DMSO extracts on Sep‐Pak C18 Plus and then Silica Plus cartridges, and determination of the BaP in the isolated extract by HPLC with fluorescence detection. Detection and quantification limits were 0.049 μg/l (0.024 μg/kg of LSLS) and 0.089 μg/l (0.045mg/kg), respectively. Recovery (87%) and CV% (≤1.5) were satisfactory.