Intrinsic ADP-Ribose Transferase Activity versus Levels of Mono(ADP-Ribose)Protein Conjugates in Proliferating Ehrlich Ascites Tumor Cells

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Abstract
Transition of proliferating Ehrlich ascites tumor cells (3 days after transplantation) to the non-proliferating status (8-14 days after transplantation) was associated with an increase in total mono (ADP-ribose) protein conjugates. This increase was largely confined to the NH2OH-resistant subfraction. When the amounts of mono(ADP-ribose) conjugates from 20% trichloroacetic acid precipitates were compared with those from 5% perchloric acid precipitates, no significant differences were seen. This fact excludes histone H1 as a major mono(ADP-ribose) acceptor in vivo in these cells. Transition to the resting state was also associated with a small decrease in NAD levels and with no significant changes of total ADP-ribose transferase activity. Intrinsic ADP-ribose transferase activity as expressed in permeabilized cells was increased, being correlated with the changes in the level of the NH2OH-resistant mono(ADP-ribose) protein conjugates. Alterations in intrinsic transferase activity may, in general, indicate similar alterations in major subfractions of ADP-ribose conjugates. Intrinsic ADP-ribose transferase activity exhibited an inverse relationship to ornithine decarboxylase activity.

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