Partial purification of a double-stranded RNA specific ribonuclease (RNAse D) from Krebs II ascites cells
- 1 August 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (8) , 2055-2066
- https://doi.org/10.1093/nar/3.8.2055
Abstract
In a search for eucaryotic enzymes which might process the heterogenous nuclear RNA (HnRNA) from animal cells into messenger RNA, a ribonuclease called RNAse D analogous to E. coli RNAse III in its ability to cleave specifically synthetic or viral double-stranded polyribonucleotides has been detected and extensively purified from the cytosol of Krebs II mouse ascites cells. The purification procedure involved cellular fractionation followed by DEAE and CM-cellulose chromatography and resulted in an RNAse D preparation contaminated with trace amounts of single-strand specific RNAse (equivalent to less than 0.3 ng per ml) as assayed against poly(rC). Significant levels of RNAse H activity against poly(rA) - poly(dT) were still present in these preparations.Keywords
This publication has 16 references indexed in Scilit:
- Synthesis and Processing of Nuclear Precursor-Messenger RNA in Avian Erythroblasts and HeLa CellsProceedings of the National Academy of Sciences, 1974
- Escherichiacoli RNASE III cleaves HeLa cell nuclear RNABiochemical and Biophysical Research Communications, 1974
- Ribonuclease III Does Not Degrade Deoxyribonucleic Acid-Ribonucleic Acid HybridsJournal of Biological Chemistry, 1974
- Cell-Free Translation of the Globin Message within Polydisperse High-Molecular-Weight Ribonucleic Acid of Avian ErythrocytesProceedings of the National Academy of Sciences, 1973
- Synthesis of a Large Precursor to Ribosomal RNA in a Mutant of Escherichia coliProceedings of the National Academy of Sciences, 1973
- T7 Early RNAs and Escherichia coli Ribosomal RNAs are Cut from Large Precursor RNAs In Vivo by Ribonuclease IIIProceedings of the National Academy of Sciences, 1973
- Biogenesis of mRNA: Genetic Regulation in Mammalian CellsScience, 1973
- Double-Stranded RNA as an Inhibitor of Protein Synthesis and as a Substrate for a Nuclease in Extracts of Krebs II Ascites CellsProceedings of the National Academy of Sciences, 1973
- Purification and Properties of Ribonuclease III from Escherichia coliJournal of Biological Chemistry, 1968
- AN ENDONUCLEASE FROM NEUROSPORA CRASSA SPECIFIC FOR POLYNUCLEOTIDES LACKING AN ORDERED STRUCTURE .I. PURIFICATION AND PROPERTIES OF ENZYME1965