IMMUNOCHEMISTRY OF I/I-ACTIVE OLIGOSYLCERAMIDES AND POLYGLYCOSYLCERAMIDES FROM RABBIT ERYTHROCYTE-MEMBRANES - CHARACTERIZATION OF LINEAR, DIANTENNARY, AND TRIANTENNARY NEOLACTOGLYCOSPHINGOLIPIDS

  • 1 January 1985
    • journal article
    • research article
    • Vol. 260  (8) , 4927-4935
Abstract
A triantennary ceramide pentadecasaccharide (BIrab-2) with blood group I and B-like activity and an unbranched ceramide heptasaccharide (Birab) with i- and B-like activity were isolated in high yield from rabbit erythrocyte membranes. The structures of the native substances and the products obtained after treatment with .alpha.-galactosidase (BIrab-2.alpha., Birab.alpha.) and subsequent Smith degradation (BIrab-2.alpha.SD) were determined by sugar analysis, methylation analysis, and fast atom bombardment-mass spectrometry of the permethylated derivatives. Together with the results of 1H NMR analysis structures were established for the native substances.

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