Bile pigment precursors in normal human erythrocytes

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Abstract
An anaerobic method for the study of bile pigment precursor in human erythrocytes and in crystalline human hemoglobin is described. The bile pigment precursor content of human erythrocytes was 0.17% of the contained hemoglobin. Bile pigment precursor also constituted 0.21% of crystalline human oxyhemoglobin. A fluorimetric method for measurement of bile pigments was developed. Its special advantages are: (a) it has a high sensitivity, adequate for the small quantities of pigments encountered in this problem; (b) it is specific, with minimal interference from other pigments; (c) it is not dependent on the prepn. of a pure bilipurpurin standard. The expts. provide little support for the hypothesis that early degradative changes of oxyhemoglobin are initiated in the circulating erythrocyte.

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