Identification of Lys190 as the primary binding site for pyridoxal 5′‐phosphate in human serum albumin
- 24 February 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 298 (2-3) , 266-268
- https://doi.org/10.1016/0014-5793(92)80073-p
Abstract
The covalent binding of pyridoxal 5′-phosphate (PLP) to human serum albumin (HSA) is important in the regulation of PLP metabolism. In plasma, PLP is bound to HSA at a single high-affinity and at two or more nonspecific sites. To characterize the primary PLP binding site, HSA was incubated with [3H]PLP, and the Schiff base linkage was reduced with potassium borohydride. Tryptic peptides were purified, and the major labeled peptide was sequenced. Amino acid analysis confirmed a homogeneous peptide Leu-Asp-Glu-Leu-Arg-Asp-Glu-Gly-Xaa-Ala-Ser-Ser-Ala-Lys which corresponds to residues 182–195 of HSA. The data indicate that Lys190 is the primary PLP binding site. This Lys residue is distinct from other sites of covalent adduct formation; namely, the primary sites for nonenzymatic glycosylation (Lys325) and acetylation by aspirin (Lys199)Keywords
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