Lymphocyte specificity to protein antigens. II. Fine specificity of T-cell activation with cytochrome c and derived peptides as antigenic probes.

Open Access
Publisher Website
Google Scholar
Abstract
Murine T [thymus-derived] lymphocyte specificity was determined in a system of antigen driven in vitro T cell proliferation using cytochrome c molecules from different species [bovine, horse, rabbit, mouse], their derived peptides and reconstituted hybrid proteins. Primed T cells could discriminate between peptide fragments which differed from each other at a single amino acid residue. These conclusions were substantiated by the pattern of cross-reactivity noted in the response of closely related cytochrome c proteins as well as when artificial hybrid molecules reconstituted by the covalent linkage of peptide fragments were analyzed. The pattern of specificity observed appeared to be haplotype (BDF1) dependent although similar conclusions about the fine specificity of T cells in the response to cytochrome c were obtained in other strains but associated with different residues.