Somatic embryogenesis and plant regeneration in callus from inflorescences of Hordeum vulgare X Triticum aestivum hybrids

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Abstract
Embryogenic callus cultures were obtained by culturing young inflorescence tissues of Hordeum vulgare cv. ‘PF51811’ (2x)XTriticum aestivum cv. ‘Chinese Spring’ (6x) hybrids on 2,4-D-containing N6 medium. After subculture for about 10 months the calli retained a high potentiality for somatic embryogenesis and plant regeneration. Of about 300 regenerated plants, approximately 100 were transplanted to potting soil. Eight embryoids and three regenerated plants examined had 28 chromosomes identical to the original hybrid plants, while one regenerated plant was found to be a mixploid composed of cells with 28 and 56 chromosomes. The possibility for obtaining amphiploid hybrids through tissue culture is discussed.