Purification of Shiga toxin by α‐d‐galactose‐(1 → 4)‐β‐d‐galactose‐(1 → 4)‐ β‐d‐glucose‐(1 →) receptor ligand‐based chromatography
- 4 December 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 258 (2) , 320-322
- https://doi.org/10.1016/0014-5793(89)81684-9
Abstract
A simple and rapid method for Shiga toxin purification based on specific binding to the Galα1 → 4Galβ1 → 4Glc globotrioside trisaccharide covalently linked to polyacryl/polyvinyl (Fractogel) has been developed. A cell-free sonicate-filtrate of Shigella dysenteriae type 1, strain 114Sd was passed over the globotrioside-Fractogel column, and bound toxin eluted with 6 M guanidine-HCl. A yield of 36 mg pure toxin/1 sonicate-filtrate was otained, i.e. a one step 1224-fold purification. The recovery of biologically active toxin was 87%. The toxin was purified to homogeneity as shown by native PAGE and SDS-PAGE.Keywords
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