Phage T4 infection restricts rRNA synthesis byE. coli RNA polymerase
- 1 January 1976
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 147 (3) , 291-297
- https://doi.org/10.1007/bf00582880
Abstract
RNA polymerase from T4 infected cells supplemented with E. coli σ polypeptide has a lower affinity for rRNA promoters than RNA polymerase from uninfected cells. The pattern of transcription by the phage modified polymerase is qualitatively similar to that of the vegetative polymerase in the presence of ppGpp. We suggest that E. coli polymerase holoenzyme normally exists in at least two conformational states, one with a high affinity for rRNA promoters and another with a low affinity, and that T4 infection stabilises the low affinity form.This publication has 22 references indexed in Scilit:
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