Lipase fromCandida paralipolytica

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Abstract
The extracellular lipase from Candida paralipolytica required alkaline earth metal ion as the cofactor in the reaction mixture not emulsified but dispersed by shaking, contradicting the fact that it required bile salt or anionic surfactant as the essential activator in the systems emulsified with polyvinyl alcohol, as previously reported. The two kinds of factors necessary to activate both reaction systems respectively were unexchangeable for each other. These facts would be direct evidences of the difference of interfacial nature between two substrate forms prepared from the same substrate. The zero-order reaction has been observed under the non-emulsified conditions and the activation mechanism by alkaline earth metal ions has been studied partly.

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