Purification and Characterization Of a Thyroglobulin Reactive Phytoprecipitin From Pisum sativum

3 January 1971

journal article
research article
Published by Taylor & Francis in Preparative Biochemistry

Vol. 1 (1) , 163-175
https://doi.org/10.1080/00327487108081937

Abstract

By means of paper curtain electrophoresis and polyacrylaaide gel filtration, it has been possible to isolate the thyroglobulin-reactive phytoprecipitin from pea (Pisum sativum; American pea; Russian pea) extract. An of 4.0 Svedbergs and a of 5.7×10⁻⁷ cm²/sec were found from analytical ultracentrifuge experiments and a specific volume of 0.61 was determined by pycnometry. These data yield a molecular weight of 43,000 for the phytoprecipitin and, postulating an oblong ellipsoid shape, an asymmetry factor of 12. Its electro-phoretic mobility corresponds with that of globulin. It could be shown that the phytoprecipitin attaches to the carbohydrate part of the thyroglobulin.

Keywords

This publication has 2 references indexed in Scilit:

27S Thyroid Iodoprotein
Published by Elsevier ,1965
Estimation of the Size of Antigens by Gel Diffusion Methods
Nature, 1959