The HPLC Analysis of Caffeine and Theobromine in Animal Diets

1 January 1982

journal article
research article
Published by Taylor & Francis in Journal of Liquid Chromatography

Vol. 5 (3) , 585-589
https://doi.org/10.1080/01483918208066916

Abstract

A method is described for the analysis of added caffeine and theobromine in rabbit diets using HPLC [high-pressure liquid chromatography] with samples extracted in CHCl3 and interferences eliminated with a Sep-pak. This method has good accuracy and precision but is not suitable for matrices where the methylxanthines exist as an integral part of the matrix (e.g., foods).

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