Kinetic features of ascorbic acid oxidase after partial deglycation
- 1 December 1989
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 264 (2) , 601-604
- https://doi.org/10.1042/bj2640601
Abstract
By means of specific exoglycosidases, sugars have been removed under non-denaturing conditions form ascorbic acid oxidase (AAO), different deglycation schedules being followed. Our results indicate that deglycation clearly affects the kinetic features of AAO, leading to an increase of ''affinity'' and ''catalytic ability'' of the enzymic forms so generated. A better exposure of the catalytic-site residues could be supposed to occur upon treatment with exoglycosidases. This is supported by the three-dimensional X-ray structure of zucchini (Cucurbita pepo medullosa; courgette) AAO.This publication has 11 references indexed in Scilit:
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