Relocating a gene for herbicide tolerance: A chloroplast gene is converted into a nuclear gene
- 1 January 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (2) , 391-395
- https://doi.org/10.1073/pnas.85.2.391
Abstract
The chloroplast gene psbA codes for the photosynthetic quinone-binding membrane protein QB, which is the target of the herbicide atrazine. This gene has been converted into a nuclear gene. The psbA gene from an atrazine-resistant biotype of Amaranthus hybridus has been modified by fusing its coding region to trancription-regulation and transit-peptide-encoding sequences of a bona fide nuclear gene. The constructs were introduced into the nuclear genome of tobacco by using Agrobacterium tumor-inducing (Ti) plasmid system, and the protein product of nuclear psbA has been identified in the photosynthetic membranes of chloroplasts. Recovery of atrazine-tolerant transgenic plants shows that the product of the transplanted gene functions in photosynthesis. These experiments show that it is possible to modify chloroplast-gene-specified functions via nuclear-genome transformation and also raise evolutionary questions.This publication has 20 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- The topology of a membrane protein: The orientation of the 32 kd Qb-binding chloroplast thylakoid membrane proteinCell, 1986
- A mitochondrial presequence can transport a chloroplast-encoded protein into yeast mitochondria.Journal of Biological Chemistry, 1986
- Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylaseNature, 1985
- Inheritance of Functional Foreign Genes in PlantsScience, 1984
- Molecular Basis of Herbicide Resistance in Plants in Amaranthus hybridusScience, 1983
- The maize chloroplast genes for the β and ε subunits of the photosynthetic factor CF1are fusedNucleic Acids Research, 1982
- Studies on the Reconstitution of O2-Evolution of ChloroplastsPlant Physiology, 1982
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Evolution of Organelles and Eukaryotic GenomesScience, 1975