Abstract
The biosynthesis of Bacitracin was shown to involve the incorporation of radioactive phenylalanine, leucine, aspartic acid, glutamic acid, onithine, lysine, and histidine. The amount of incorporation of the labelled amino acids into Bacitracin, which was isolated by column chromatography, corresponds to the amount of microbiologically determined antibiotic formed. The formation of the antibiotic is stimulated by additions of glucose, isoleucine, cysteine and an unidentified factor derived from soybean protein.

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