Nicotinic acetylcholine receptor-like molecules in the retina, retinotectal pathway, and optic tectum of the frog
Open Access
- 1 February 1989
- journal article
- research article
- Published by Society for Neuroscience in Journal of Neuroscience
- Vol. 9 (2) , 565-573
- https://doi.org/10.1523/jneurosci.09-02-00565.1989
Abstract
Forty-two monoclonal antibodies (mAbs) generated against nicotinic acetylcholine receptors (AChRs) from electric organ were tested for their ability to cross-react in the optic tectum of the frog Rana pipiens. Twenty-eight of the mAbs tested (67%) bound to the optic neuropil of the tectum as revealed by immunoperoxidase cytochemistry. The pattern of peroxidase stain for cross-reacting mAbs corresponded in position to a subset of the retinotectal projections. Electron microscopic examination revealed that peroxidase reaction product was associated with the surface of vesicle-containing profiles but not with synaptic sites. Removal of one retina resulted in the loss of immunoreactivity in the contralateral tectum. AChR-like immunoreactivity was also associated with the optic tract and optic nerve and with retinal ganglion cells. These results indicate that some classes of retinal ganglion cells bear AChR-like molecules on their surface. The existence of these molecules on ganglion cell axons and terminals seems the most likely explanation for the AChR-like immunoreactivity present in the tectum.This publication has 36 references indexed in Scilit:
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