SYNTHESIS OF ?-FETOPROTEIN BY RAT ASCITES HEPATOMA CELLS
- 1 August 1975
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 259 (1) , 29-36
- https://doi.org/10.1111/j.1749-6632.1975.tb25399.x
Abstract
AH-66 rat ascites hepatoma cells incorporated [14C]leucine into the AFP fraction. In a cell-free system, hepatoma ribosomes were found to be active in AFP synthesis whereas the supernatant fraction from hepatoma had no specific effect on AFP production. The amount of [14C]leucine incorporated in AFP by membrane-bound polysomes was 20 to 90 times higher than that by free polysomes, suggesting that AFP is mainly synthesized on membrane-bound polysomes. DBcAMP inhibited the growth of hepatoma cells. However, the incorporation of [14C]leucine into the AFP fraction as well as into total proteins was stimulated by DBcAMP.Keywords
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