Human milk stimulates DNA synthesis and cellular proliferation in cultured fibroblasts.
- 1 October 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (10) , 5057-5061
- https://doi.org/10.1073/pnas.75.10.5057
Abstract
Human milk contains a mitogenic factor that stimulates DNA synthesis and cell division in mouse and human fibroblasts in vitro. Milk at a concentration of 1% (vol/vol) is as active in stimulating DNA synthesis as is 5% (vol/vol) human serum and 10% (vol/vol) calf serum. The mitogenic activity of human milk is destroyed by incubation with trypsin and chymotrypsin. However, neither urea, guanidine hydrochloride, dithiothreitol, nor exposure to pH 1 will inactivate the milk-derived growth factor. Gel filtration and isoelectric focusing indicate that the mitogenic activity of human milk has a MW between 14,000-18,000 and an isoelectric point between 4.4-4.7.This publication has 6 references indexed in Scilit:
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