On the Enzymic Preparation of Decarboxylated Adenosylmethionine.

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Abstract
A method is described for enzymic preparation of S-methyladenosylhomocysteamine (decarboxylated adenosylmethionine) with the aid of Mg-activated adenosylmethionine decarboxylase [EC 4.1.1.50] partially purified from cells of Escherichia coli. The procedure involves chromatography on Dowex 50-H+ column, followed by preparative paper electrophoresis and rechromatography on Dowex 50-H+. The procedure yields a pure preparation which is suitable for measurements of the activity of spermidine and spermine synthases.
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