DNA polymerase of a basidiomycete fungus, Coprinus cinereus

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Abstract
DNA polymerase activity was studied in C. cinereus, a basidiomycete fungus. Only 1 form of the enzyme was demonstrated, whether by affinity or ion-exchange chromatography; this enzyme has a MW of 185,000 on Sephadex G-200 and was inhibited by mercaptoethanol. Coprinus, a representative of the most advanced type of the filamentous fungi, resembles other eukaryotic microorganisms in its lack of a mammalian .beta.-type DNA polymerase. The properties of the polymerase are compared with Ustilago and an Ascomycete yeast and resemble closely the yeast polymerase A in Mg2+ requirements and template preference.