Abstract
Summary Latrodectus tredecimguttatus antivenin produced in Yugoslavia proved effective in protecting 20-gram white mice from lethal doses of venoms of L. mactans and L. hasseltii. In all potency tests intraperitoneal injections of venom were followed in 30 minutes by intraperitoneal injections of antivenin. Test animals were observed for a 24 hour period, after which time results were recorded. Death and survival were regarded as endpoints. Amounts of venom were expressed as portions of the contents of one venom gland. One-fourth the contents of one venom gland constituted the minimum certain lethal dose of L. mactans venom for white mice. Complete protection against effects of two certain lethal doses of mactans venom was afforded by amounts of antivenin as low as 0.1 cc. of a 1:1 dilution.

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