Study and Optimization of Citrus Flavanone and Flavones Elucidation by Reverse Phase HPLC with Several Mobile Phases: Influence of the Structural Characteristics

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Abstract
A high-performance liquid chromatographic procedure for the analysis of the different flavonoids found in Citrus aurantium tissue extracts has been developed. It employs a C18 reverse phase column and an elution isocratic-gradient system with a mixture of water, methanol, acetonitrile and acetic acid. We make an exhaustive description of the optimisation process by studying the quantitative chromatographic parameters: k1, w, a, N, HETP and R. The use of different extraction solvents for flavonoids in Citrus aurantium tissues was also studied, DMSO presenting the best results. Twelve compounds were detected, isolated and their spectral structures characterized.