Active hydrogen by chemical ionization mass spectrometry

Publisher Website
Google Scholar
Abstract
A means of determination of active hydrogen of hydroxyl, carboxyl, sulfhydryl, amino, amido and sulfonamido groups by chemical ionization mass spectrometry using ammonia and trideuterioammonia as reagent gases is described. The method is based on exchange of active hydrogen for deuterium occurring during the chemical ionization process using trideuterioammonia, with comparison of m/z for ammonium adduct [M+NH4]+ and trideuterioammonium adduct [M‐nH+nD+ND4]+, or of protonated molecular ion [M+H]+ and deuteronated molecular ion [M‐nH+nD+D]+ yielding the number of active hydrogens. Applications have been made to several classes of biologically important compounds.