Adenylate cyclase activity of bovine spermatozoa during maturation in the epididymis and the activation of sperm particulate adenylate cyclase by GTP and polyamines

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Abstract
The adenylate cylase activity of bovine caput and cauda epididymal spermatozoa was measured in intact- and in broken-cell preparations. Cyclase activity was 4-fold greater in caput than in cauda cells and total cyclase activity in broken-cell preparations was 305 times greater than that in intact cells. A particulate fraction derived from sonically disrupted caput spermatozoa was used to study the effects of compounds that might be physiologically important modulators of adenylate cyclase. Activity was stimulated by GTP, 5-guanylyl imidophosphate and by the polyamines, spermine and spermidine.