Tight association of DNA polymerase .ALPHA. with granular structures in the nuclear matrix of chick embryo cell: Immunocytochemical detection with monoclonal antibody against DNA polymerase .ALPHA..
- 1 January 1984
- journal article
- research article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 9 (1) , 83-90
- https://doi.org/10.1247/csf.9.83
Abstract
Immunofluorescent methods using a monoclonal antibody against chick DNA polymerase .alpha. and a rabbit antibody against chick DNA polymerase .beta. demonstrated that both DNA polymerases .alpha. and .beta. are present mainly in nuclei of cultured chick embryo cells. Fluorescence produced by anti-DNA polymerase .beta. was distributed evenly in the nucleus. Cells first were treated with Nonidet P-40, followed by treatment with 50 .mu.g/ml pancreatic DNase and 2 M NaCl to prepare the nuclear matrix. Fluorescence produced by anti-DNA polymerase .alpha. was still detectable in the granules after these treatments, but most of the fluorescence produced by anti-DNA polymerase .beta. disappeared. A part of DNA polymerase .alpha. is tightly bound to a special structure present in the nuclear matrix which presumably is the DNA replication machinery.Keywords
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