Cellulase immobilization on Fe3O4 and characterization
- 15 January 1989
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 33 (3) , 321-326
- https://doi.org/10.1002/bit.260330311
Abstract
A successful procedure for attaching cellulase to 45 μm iron oxide particles with a high‐molecular‐weight (1000) ligand has been developed. Mass and activity balances were calculated for immobilized enzymes with different loadings. The highest specific activity of the immobilized enzyme was 5.9 mmol glucose/g bound protein/h. The efficacy of retaining enzymatic activity was 128%. The optimum pH was 5.5 compared to 4.0 of the free enzyme. The half‐life of the IMC was extended to 272 h compared to 0.77 h of the free enzyme.Keywords
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