New Method for Large-Scale Preparation of Covalently Closed λ DNA Molecules
Open Access
- 1 November 1974
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 14 (5) , 1104-1107
- https://doi.org/10.1128/jvi.14.5.1104-1107.1974
Abstract
A combination of mutations in bacteriophage λ and its host Escherichia coli K-12 provides a convenient system for the isolation of large quantities of covalently closed circular DNA molecules. We describe two procedures for the large scale preparation of λ DNA in the duplex circular form.Keywords
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