Ribonucleic acid synthesis by Escherichia coli C3000/L after infection by the ribonucleic acid coliphage ZIK/1, and properties of coliphage-ZIK/1 ribonucleic acid
- 1 October 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 97 (1) , 17-26
- https://doi.org/10.1042/bj0970017
Abstract
A method is described for the preparation and purification of the RNA from the RNA coliphage ZIK/1. Some of the physical characteristics and infective properties of coliphage-ZIK/1 RNA were examined. A method is also described for examining the type and quantity of RNA synthesized after bacteriophage infection. Ribosome synthesis was decreased 15 min. after bacteriophage adsorption, bacteriophage RNA was synthesized from 15 min. to 120 min. after adsorption and intracellular bacteriophages appeared 40 min. after adsorption. Cell lysis commenced 60 min. after adsorption, and was half complete 20 min. later and 90-95% complete 120 min. after adsorption. Cell division continued until 40 min. after bacteriophage adsorption. Bacterial ribosomes were conserved during the infective process. Intracellular bacteriophage RNA has sedimentation coefficient 28 s but after cell lysis it has sedimentation coefficient 10-5 s.This publication has 22 references indexed in Scilit:
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