Comparison of pH and Ionic Strength Dependence of Interactions between Monoclonal Antibodies and Bovineβ-Lactoglobulin
- 1 January 1996
- journal article
- research article
- Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry
- Vol. 60 (1) , 25-29
- https://doi.org/10.1271/bbb.60.25
Abstract
A panel of 13 monoclonal antibodies (mAbs) against distinct determinants on bovine beta-lactoglobulin, a model protein antigen, were examined and compared for their ability to bind and desorb from the antigen at differing pHs and ionic strengths by an enzyme-linked immunosorbent assay and elution assay. Among them, mAb 61C1 was found to be highly sensitive to the pH, and 3 in 4 mAbs directed to the region 42-56 also strongly depended on the change in ionic strength. Because of the large proportion of charged amino acid residues in the region 42-56, the electrostatic forces are considered to be more predominant than the hydrophobic interactions in the latter antigen-antibody reactions, thereby resulting in their high sensitivity to the ionic strength.Keywords
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