Direct expression in Escherichia coli and characterization of bovine adrenodoxins with modified amino‐terminal regions
- 6 April 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 300 (3) , 208-212
- https://doi.org/10.1016/0014-5793(92)80847-a
Abstract
Four forms of bovine adrenodoxin with modified amino-termini obtained by direct expression of cDNAs in Escherichia coli are Ad(Met1), Ad(Met−1), Ad(Met−12), and Ad(Met6). The shoulder numbers represent this site of translation initiator Met at the amino-termini. The adrenodoxins, except for Ad(Met−1), were purified from the cell lysate and the ratios of A 414-to-A 276 of the purified proteins were over 0.92. NADPH-cytochrome c reductase activities of the three forms of adrenodoxin in the presence of adrenodoxin reductase were the same as that of purified bovine adrenocortical adrenodoxin. However, as cytochrome P-450SCC reduction catalyzed by Ad(Met0) was about 60% or that by Ad(Met1), the contribution of the amino-terminal region for the electron transfer or binding to cytochrome P-450SCC would need to be considered.Keywords
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