Thermal stability of the deoxyribonucleic acid hybrids between the proteolytic strains of Clostridium botulinum and Clostridium sporogenes

Abstract
Percentage lesion of the deoxyribonucleic acid (DNA) hybrids of 19 strains of proteolytic Clostridium botulinum and Clostridium sporogenes was determined by the thermal stability method. The maximum noncomplementary lesion was 6–9%, but in most instances the lesions were much less than 6 or 9%. The results indicated that the DNA hybrids of C. botulinum and C. sporogenes tested were homologous, and that some of these toxic and nontoxic strains are closely related.

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