Binding of mercury(II) to poly(dA-dT) studied by proton NMR
- 5 January 1982
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 21 (1) , 62-66
- https://doi.org/10.1021/bi00530a012
Abstract
The binding of Hg(II) to poly(dA-dT) was examined with proton NMR spectroscopy. Addition of HgCl2 between r (Hg2+/nucleotide) = 0 and 0.25 results in loss of the exchangeable imino N3H resonance of thymine, indicating preferential binding at this site. The nonexchangeable base resonances AH8, AH2 and TH6 shift their intensity downfield in a cooperative manner, indicating complexation which is slow on the NMR time scale and changes in the polymer conformation upon binding. At r = 0.25, the polymer is cross-linked and an increase in temperature does not result in denaturation of the polymer, as evidenced by the thymine proton resonance chemical shifts. The chemical shifts of the AH2 and T(CH3)5 base resonances allow some general conclusions to be made about the stereochemistry of this complex.This publication has 32 references indexed in Scilit:
- A circular dichroism study on the structure of DNA and the nucleosomal core particle using Hg(II) and Ag(I)Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1980
- Electric dichroism and sedimentation velocity studies of DNA-Hg(II) and DNA-Ag(I) complexesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1980
- On the conformational dependence of the proton chemical shifts in nucleosides and nucleotides. II. Proton shifts in the ribose ring of purine nucleosides as a function of the torsion angle about the glycosyl bondJournal of Theoretical Biology, 1977
- On the conformational dependence of the proton chemical shifts in nucleosides and nucleotides. I. Proton shifts in the ribose ring of pyrimidine nucleosides as a function of the torsion angle about the glycosyl bondJournal of Theoretical Biology, 1977
- On the atomic or “local” contributions to proton chemical shifts due to the anisotropy of the diamagnetic susceptibility of the nucleic acid basesBiochemical and Biophysical Research Communications, 1976
- Interactions of mercury and copper with constitutive heterochromatin and euchromatin in vivo and in vitroBiochemistry, 1976
- Mercury binding to nucleic acids. Crystal and molecular structures of 2:1 complexes of uracil-mercuric chloride and dihydrouracil-mercuric chlorideBiochemistry, 1971
- Interaction of mercuric ions with tomato bushy stunt virusBiochemistry, 1970
- PREPARATION AND PROPERTIES OF NATIVE CRAB dATProceedings of the National Academy of Sciences, 1965
- The Reaction of Mercury(II) with NucleosidesJournal of the American Chemical Society, 1963