Monoclonal Antibodies against the Major Internal Protein, p27, of a Psoriasis-Associated Retrovirus-Like Particle
- 1 August 1988
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 28 (2) , 195-202
- https://doi.org/10.1111/j.1365-3083.1988.tb02431.x
Abstract
Four murine monoclonal antibodies (MoAb) were raised against p27 isolated from psoriatic scale. The MoAb recognized the antigen on a subfraction (0.1-1%) of psoriatic lymphocytes as well as in skin biopsies from psoriatic patients. In contrast, the antigen could not be detected on lymphocytes or in skin biopsies from healthy controls. A sandwich ELISA assay using the MoAb for detection of p27 is described. This method, together with a competition antibody-binding assay for distinction of epitopes, demonstrates that the MoAb recognize four different antigenic determinants on p27.Keywords
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