High Performance Liquid Chromatography of Enkephalin and Endorphin Peptide Analogs

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Abstract
Reverse phase systems are presented which utilize a μ alkylphenyl column and ammonium acetate buffered aqueous acetonitrile mobile phases to separate mixtures of enkephalin and endorphin peptide analogs. High pressure liquid chromatographic separations of mixtures of enkephalin diastereomers as well as mixtures of other closely similar analogs have been developed. Endorphin analogs were observed to be quite hydrophobic and required mobile phases containing 40% or more acetonitrile for reasonable elution times. The enkephalin analogs by comparison required 20% or more acetonitrile. Detection at both 254 and 280 nm was useful in recognizing the important peaks in the elution profile.