SOME EFFECTS ON LEUKEMIC B-LYMPHOCYTES OF ANTIBODIES TO DEFINED REGIONS OF THEIR SURFACE-IMMUNOGLOBULIN

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 32  (4) , 549-557
Abstract
During the growth of the L2C leukemia in strain 2 guinea-pigs there is excreted in the urine a monoclonal L chain of .lambda. class. This probably arises from synthesis by the leukemic cells of a small molar excess of the L chains incorporated into their surface Ig[immunoglobulin]M. Antibodies to this L chain were raised in rabbits and separated by immunosorption into those directed against idiotypic determinants (anti-Id), and those against determinants also present on other .lambda. chains (anti-.lambda.). Both antibodies react with L2C cells via the surface Ig. Anti-Id is specific for L2C cells while anti-.lambda. also reacts with 7% of normal nodal lymphocytes. L2C cells are agglutinated strongly by anti-Id and weakly by anti-.lambda.. Both antisera inhibit migration of the cells, and both can kill them by invoking complement or K[antibody-dependent killer]-cell cytotoxic mechanisms. The potential of anti-idiotype sera for identifying and specifically attacking neoplastic B [bone marrow-derived] lymphocytes was emphasized.