The complete genome of Bacillus subtilis: from sequence annotation to data management and analysis

Open Access
Publisher Website
Google Scholar
Abstract
The completion of the entire 4.2‐Mb genome sequence of the Gram‐positive bacterium Bacillus subtilis has been a milestone for biological studies on this model organism. This paper describes bioinformatics work related to this joint European and Japanese project: methods and strategies for gene annotation and detection of sequencing errors, using an integrated cooperative computer environment (Imagene); construction of a specialized database for data management and a WWW server for data retrieval (SubtiList); DNA sequence analysis, yielding striking results on oligonucleotide bias, repeated sequences, and codon usage, all landmarks of evolutionary events shaping the B. subtilis genome.