In vitro methylation of the elongation factor EF‐Tu from Escherichia coli
- 12 November 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 193 (1) , 17-21
- https://doi.org/10.1016/0014-5793(85)80070-3
Abstract
The in vitro methylation of the elongation factor EF-Tu from Escherichia coli was investigated. The methylation of newly synthesized EF-Tu was obtained using λrifd18 DNA as template and S-adenosyl [methyl-3H]methionine as methyl donor. About 3 mol methyl residues were incorporated for every 10 mol EF-Tu synthesized. Analysis of the nature of the methyl-containing residues by protein hydrolysis followed by paper chromatography showed that both mono- and dimethyllysine were present. The methylation of EF-Tu was also studied separately from its synthesis by using cell-free systems with artificially undermethylated components.Keywords
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