A human gallbladder adenocarcinoma cell line

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Abstract
A cell strain (FU‐GBC‐1) was established from cancerous ascites of a 68‐year‐old male patient with well‐differentiated adenocarcinoma of the gallbladder. By light and electron microscopy, the cultured cells showed the morphologic features of adenocarcinoma characterized by gland‐like structures, intracellular microcystic spaces, and mucous production. Immunoperoxidase stains showed that FU‐GBC‐1 cells expressed several epithelial tumor antigens including CA 19‐9, carcinoembryonic antigen (CEA), and epithelial membrane antigen (EMA). The cell strain has been in continuous culture up to passage 44 for 1 1/2 years, with the population doubling time of 120 hours. The cytogenetic analysis by a G‐band technique showed a constant loss of chromosome Y in FU‐GBC‐1 cells. The modal chromosome number at passage 12 was 82 with a range of 77 to 85. Flow cytometry with an ethidium bromide technique additionally confirmed aneuploid DNA content (4C) in the cultured cells at passage 12 and 35. Inoculation of FU‐GBC‐1 cells into the dermis of BALB/c nude mice produced transplantable adenocarcinoma identical to the original tumor. Because no continuous cell lines of the well‐differentiated type of gallbladder adenocarcinoma have been reported in the literature currently, the newly established cell strain we report may yield a useful system for studying the morphologic and biologic characteristics of gallbladder adenocarcinoma.

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