Preparation of protoplasts of group H streptococci (Streptococcus sanguis).

  • 1 January 1975
    • journal article
    • Vol. 29  (1) , 90-3
Abstract
Stable protoplasts of several strains of group H streptococci (Streptococcus sanguis) can be prepared by use of group C streptococcal phage-associated lysin in the presence of 30% raffinose. Sucrose cannot be substituted for raffinose. Protoplast formation did not require the addition of Mg2+; however, this cation enhanced their stability. Some other strains, also presumptive group H streptococci, were not sensitive to phage-associated lysin.